Ukukhulisa isithintelo esisebenzayo seGazi-leBongo

Ngabasebenzi beBongo kunye nokuziphatha

Abaphandi bekhokelwa ngu-2016 BBRF Umphandi oselula u-Ethan Lippmann, Ph.D., banike ingxelo yokuba baphumelele "ekwakheni" izicwili zemithambo ezisebenza njengombilini obalulekileyo okhuselayo wobuchopho, obizwa ngokuba ngumqobo wegazi nengqondo. Isithintelo sisebenza njengesefa ekhethiweyo, igcina iimolekyuli ezinkulu kubandakanya ibhaktiriya ngaphandle kwengqondo kunye nolusu lomqolo, kodwa ivumela ioksijini, iswekile, kunye nezinye izinto ezibalulekileyo ukuba zingene.

Umsebenzi, owenziwe kwiDyunivesithi yaseVanderbilt kwaye wapapasha ngoFebruwari 14, 2019 kwiiNgxelo zeSeli yeStem, kufuneka uncede ukukhawulezisa ukuguqulelwa kwezimvo zesayensi kuphando ngengqondo.

Ngelixa iinkcubeko ezibini-zeseli ezinobuchopho-zeseli zikhulile ngaphambili, eli lixesha lokuqala imodeli enamacala amathathu esebenza njengomqobo wegazi lomntu nengqondo eyenziweyo. Imodeli ikhulile ukusuka kwiiseli ezisampulu ukusuka kwisithambo somntu esinyanzeliswa ukuba siphuhle njengohlobo lweseli ekhethekileyo esisiseko somqobo wegazi nengqondo. Emva koko badityaniswe kwimatrix enamacala amathathu esebenza njengesafu.

Inkqubo yokuphinda yenziwe ngokutsha iiseli, eyaba nguvulandlela kuphando lobuchopho ngabaxhasi beBBRF nabanye kule minyaka ilishumi idlulileyo, ibizwa ngokuba yi-iPSC, emele itekhnoloji ye "cell pluripotent stem cell". Inezicelo ezininzi kumayeza onke, ngakumbi ekudalweni kweentlobo ngeentlobo ze "organoids" -eziphilayo, iinkcubeko ezinemilinganiselo emithathu yeeseli ezidityanisiweyo ukuze ziphinde zikhule njengeentlobo zeseli ezithile kumalungu ohlukeneyo omzimba. Enye indlela ethembisayo eya phambili kuvavanyo lweziyobisi kunye nophando lwezifo ilele ekwenzeni iimodeli ze-organoid zamalungu abantu, ukumisela ukusebenza kunye namandla amayeza.

Ngelixa abaphandi bezamile ngeendlela zobuchopho ezingaqhelekanga, indlela entsha yokuphinda yenziwe izakhiwo ezithatha indima yesithintelo segazi lengqondo yomntu, ukuba ibandakanyiwe kwingqondo ye-organoids, inokuzisa inzululwazi inyathelo elikhulu kufutshane nokwenza "ubuchopho kwisitya" ngokuthembekileyo. phindaphinda kokubini ukwakheka kunye nokusebenza kweyona nto yengqondo yomntu, okanye iinxalenye zazo.

Ukuphinda phinda isithintelo se-endothelial kwingqondo ye-organoids kubalulekile, kuba ingqondo kufuneka ikhuselwe kwizinto ezisegazini.

Isithintelo segazi nengqondo sikhula "ukuvuza" kwizigulo ezithile, kubandakanya izifo ezithile zemithambo-luvo kubandakanya i-ALS kunye nokuxhuzula. Kukwenzeka ngakumbi xa ukudumba emzimbeni kufikelela kumanqanaba aphezulu. Le inokuba yenye yeendlela apho iimolekyuli ezivuthayo zingena kwingqondo kunye nomsebenzi ophazamisayo oqhelekileyo, umzekelo kwi-multiple sclerosis.